Salmonella Chromogenic Medium For Rapid Isolation And Identification of Salmonella, 1L

Salmonella Chromogenic Medium
(Chromogenic Salmonella Agar)
 
Usages:
For rapid isolation and identification of Salmonella.
 
Principle:
Ability antibacterial agents against Gram-positive bacteria, and enhance the medium inhibit bacteria; peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; sodium chloride maintains osmotic equilibrium; agar as culture medium coagulant ; mixing pigment with Salmonella and coliforms, respectively corresponding to the occurrence of a specific enzyme reaction, hydrolysis of the substrate, the release of the color groups in the pale yellow tablet Salmonella produce magenta colonies, coliform produce blue-green colonies .
 
Formulation (per liter):
Peptone :19.6g
Yeast extract powde:r 3g
Sodium chloride: 5g
Bacteriostatic agent :1.5g
Agar :12g
Mixed Pigment: 6.4g
Final pH 7.0 ± 0.2
 
How to use:
1.Suspend 47.5g the product, dissolved in 1000ml of distilled water, mixed and heated to 100 ℃, stirring constantly until the regular press completely dissolved (can not be heated to high temperatures sustainely, it is recommended not to repeat the boiling solvent), without autoclaving , cooled to 50 ℃, pour on sterilized dishes.
2.Prepare fluid sample and enrichment broth according to the national standard, SN standards, FDA standards or other sample preparation methods.
3.Two-step enrichment method was recommended:
(1) pre-enrichment: place 25g of the treated sample in 225mL buffered peptone water and mixed uniformly at 37 ℃ cultivating for 6 ~ 8h;
(2) selective enrichment: Take 1mL of Pre-enrichment broth and adding to 9mL of four brilliant green broth, sodium sulfur enrichment broth (TTB), and after mixing 37 ℃  cultivating for 18 ~ 24h;
4.Take one loop of the enrichment broth with inoculation loop, streaked onto Salmonella color tablet, placed in 36 ± 1 ℃ cultured for 18-24 hours.
5.Observe the results.  picked magenta color, flat and transparent, smooth edges, about 2mm in diameter suspicious colonies for further testing on the plate.
6. MicrogenTMGN-ID or API20E or biochemical test tube was used for suspected biochemical identification of Salmonella colonies.
 
Quality control:
This product appears light yellow after the pour on plate, these strains were inoculated after 36 ± 1 ℃ cultured 18-24h growth in the following table.
Bacteria name            bacteria NO.          growth situation         feature
Salmonella typhimurium     CMCC50115         good                magenta colonies
Escherichia coli            ATCC25922          good              blue-green colonies
Proteus mirabilis           CMCC (B) 49005      good              colorless colonies
Enterococcus faecalis       ATCC29212          suppressed           -----
 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light.
 
Specifications: Configurable 1L of Medium .